Abstract：

The root of legume plant plays crucial role in nitrogen fixation. However,the transcriptomes of different cell types of legume root and their functionsremain largely unknown. Here, we performed single-cell RNA sequencingand profiled more than 22,000 single cells from root tips of Lotusjaponicus,a model species of legume. We identified seven clusters corresponding to seven major cell types, which were validated by in situhybridization. Further analysis revealed regulatory programs includingphytohormone and nodulation associated with specific cell types,andrevealed conserved and diverged features for the cell types. Our resultsrepresent the first single-cell resolution transcriptome for legume root tipsand a valuable resource for studying the developmental and physiologicalfunctions of various cell types in legumes.

Key Words:

Genome, Legume, Lotus japonicus, Single cell RNA-seq